ResearchOverview

The Shaughnessy Lab investigates the physiological mechanisms that support epithelial transport processes, endocrine signaling pathways, and environmental adaptation, across diverse vertebrate systems and macroevolutionary timescales. Our work integrates molecular, cellular, and whole-organism approaches, with a particular emphasis on ion transport, hormonal signaling, and stress physiology. Research in the lab spans both fundamental and applied questions, and is organized around three complementary themes: Comparative Physiology, which examines the evolution and regulation of physiological systems across taxa; Applied Physiology, which addresses conservation or industry needs by contributing physiological insights; and Translational Physiology, which focuses on epithelial transport and CFTR function in the context of human disease.

ComparativePhysiology

Our research in this theme is centered on processes of environmental physiology and the endocrine programs that regulate them. We integrate in vivo, ex vivo, and in vitro approaches and focus our attention primarily to processes of ionoregulation, stress, and thermal tolerance. Although the majority of our studies have centered on fishes, past and ongoing work also includes studies on crustaceans and amphibians. Given that we work heavily on basal lineages of fishes (i.e., hagfishes, lampreys, elasmobranchs, and non-teleosts), a major emphasis of this work that has emerged is in understanding how complexity in physiological processes evolved throughout the radiation of vertebrates, including how endocrine signaling systems or epithelial transport processes gained or lost function through genome evolutionary events. This research is deeply integrated in a broader community of comparative physiology and endocrinology, and we regularly present our work at meetings such as the International Congress on the Biology of Fish (ICBF), the North American Society for Comparative Endocrinology (NASCE), as well as other meetings less frequently.

Comparative | Epithelial Ion Transport and Osmoregulation

This research focuses on how aquatic vertebrates regulate salt and water balance through epithelial ion transport. We investigate the function of ionocytes (i.e., specialized cells that mediate epithelial ion movements) in the gill and intestinal tract and the hormonal signals that modulate their presence and activity during developmental and environmental changes. Our investigations combine organismal experiments with molecular, histological, and electrophysiological analyses to examine ionoregulatory mechanisms. By combining molecular and functional approaches in both model and non-model fishes, we explore the diversity and plasticity of osmoregulatory physiology in vertebrates. These comparative studies aim to understand how varied ion transport strategies enable ecological transitions (e.g., movement between fresh and salt water) and evolved throughout the radiation of vertebrates.

Ongoing Projects

Selected Papers

A Cftr-independent, Ano1-rich seawater-adaptive ionocyte in sea lamprey gills

A Cftr-independent, Ano1-rich seawater-adaptive ionocyte in sea lamprey gills

J Exp Biol, 2025

Shaughnessy CA, Hall DJ, Norstog JL, Ferreira-Martins D, Barany A, Regish AM, Breves JP, Komoroske LM, McCormick SD

Endocrine control of gill ionocyte function in euryhaline fishes

Endocrine control of gill ionocyte function in euryhaline fishes

J Comp Physiol B, 2024

Breves JP, Shaughnessy CA

Functional characterization and osmoregulatory role of gill Na+/K+/2Cl- cotransporter (NKCC1) in sea lamprey (Petromyzon marinus), a basal vertebrate

Functional characterization and osmoregulatory role of gill Na+/K+/2Cl- cotransporter (NKCC1) in sea lamprey (Petromyzon marinus), a basal vertebrate

Am J Physiol Regul Integr Comp Physiol, 2020

Shaughnessy CA, McCormick SD

Comparative | Evolution of Neuroendocrine Signaling Pathways

This research investigates the functional evolution of neuroendocrine signaling pathways in early vertebrates. We focus primarily on the hypothalamus-pituitary-adrenal/interrenal (HPA/HPI) axis, including the melanocortin (Acth/Msh) and corticotropin-releasing hormone (Crh) signaling systems. We examine how functional diversity of ligands and receptors in these systems emerged across the radiation of vertebrates. Our work combines manipulative physiological experiments (e.g., administration of hormones or receptor antagonists) with in vitro experimentation and molecular analyses (e.g., cloning, heterologous expression in mammalian systems, pharmacological characterization, and evolutionary genetics). Through these approaches, we explore the physiological role(s) of these neuroendocrine pathways in basal vertebrates, the evolution of receptor function (e.g., ligand-receptor specificity and cell signaling interactions), and the expansion, diversification, and specialization of endocrine gene families. Our comparative studies in basal fishes aim to further our understanding of how changes in receptor architecture and regulatory elements contributed to the emergence of endocrine complexity in vertebrates.

Ongoing Projects

Selected Papers

Melanocortin receptor divergence in lamprey

Functionally divergent melanocortin receptor subtypes and the HPI axis in sea lamprey

J Mol Endocrinol, 2025

Shaughnessy CA, Myhre VD, McCormick SD, Dores RM

Mc2r evolution in lobe-finned fish

Functional characterization of melanocortin 2 receptor (Mc2r) from a lobe-finned fish (Protopterus annectens) and insights into the molecular evolution of melanocortin receptors

Gen Comp Endocrinol, 2023

Shaughnessy CA, Le K, Myhre VD, Dores RM

HPI axis in sturgeon

Hypothalamus–pituitary–interrenal (HPI) axis signaling in Atlantic sturgeon (Acipenser oxyrinchus) and sterlet (Acipenser ruthenus)

Gen Comp Endocrinol, 2023

Shaughnessy CA, Myhre VD, Hall DJ, McCormick SD, Dores RM

AppliedPhysiology

Our applied physiology research leverages fundamental principles of comparative physiology to address real-world challenges in conservation, aquaculture, and fisheries management. We study how animals physiologically respond to environmental change and stress, and use this knowledge to inform practices that support the sustainability of natural and managed populations. This includes developing experimental tools and physiological biomarkers for assessing tolerance, performance, and resilience in native and invasive species. Our work often involves collaborations with agencies and stakeholders and seeks to produce insights that can be translated into actionable outcomes for biodiversity preservation, invasive species control, and habitat management.

Applied | Physiological Responses to Environmental Stress

This research focuses specifically on the stress component of environmental physiology, with particular emphasis on thermal stress, salinity stress, and the physiological effects of chronic stress from adverse environmental conditions. We study how endocrine systems, such as corticosteroid signaling, regulate or result from adaptive stress responses to ecological or environmental challenges. We also examine the role of hepatic metabolism as a downstream effector of corticosteroid action, particularly in mediating energy mobilization during acute and chronic stress. Our approach integrates organismal experiments (e.g., chronic stress paradigms, heat shock and CTmax trials), endocrine profiling, and molecular and biochemical analyses (e.g., Hsp expression and metabolic enzyme activities). Through these efforts, we aim to understand how stress tolerance and endocrine function vary across taxa, populations, and environmental contexts.

Ongoing Projects

Selected Papers

11-Deoxycortisol in juvenile sea lamprey

11-Deoxycortisol is a stress responsive and gluconeogenic hormone in a jawless vertebrate, the sea lamprey (Petromyzon marinus)

J Exp Biol, 2021

Shaughnessy CA, McCormick SD

Thermal tolerance in American shad

Upper thermal tolerance and heat shock protein response of juvenile American shad (Alosa sapidissima)

Estuaries Coasts, 2020

Bayse S*, Shaughnessy CA*, Regish A, McCormick SD

Brook trout thermal tolerance

Reduced thermal tolerance during salinity acclimation in brook trout (Salvelinus fontinalis) can be rescued by prior treatment with cortisol

J Exp Biol, 2018

Shaughnessy CA, McCormick SD

Applied | Physiology in Conservation and Aquaculture

This research produces and applies physiological tools to understand or predict how aquatic vertebrates respond or adapt to changing or stressful environmental conditions, particularly in conservation and aquaculture contexts. We investigate how thermal, osmotic, and seasonal stressors affect the performance and survival of native, invasive, introduced, or cultured species. Projects in this area include the integration of physiological experiments with ecological modeling to evaluate environmental limits, identify management-relevant biomarkers, and characterize intraspecific variation in physiological responses environmental pressures. This work often involves collaboration with ecologists, aquaculturists, and conservation agencies (inlcuding the Oklahoma Department of Wildlife Conservation and the Great Lakes Fishery Commission) focused on population ecology, ecophysiology, species resilience,invasive species control, environmental adaptation, or sustainable agriculture.

Ongoing Projects

Selected Papers

Frog thermal tolerance and distribution modeling

Physiological and morphological traits affect contemporary range expansion and implications for species distribution modeling in an amphibian species

J Anim Ecol, 2025

Edwards OM, Reichert MS, Ozmen L, Shaughnessy CA, Zhai L, Zhang B

Wolf eel aquaculture and osmoregulation

The isosmotic point as critical salinity limit for growth and osmoregulation, but not survival, in the wolf eel Anarrhichthys ocellatus

Fish Physiol Biochem, 2022

Shaughnessy CA, Balfry SK, Bystriansky JS

Springtime warming in sea lamprey

Juvenile sea lamprey (Petromyzon marinus) have a wide window of elevated salinity tolerance that is eventually limited during springtime warming

Can J Fish Aquat Sci, 2023

Shaughnessy CA, McCormick SD

TranslationalPhysiology

Our translational work focuses on epithelial ion transport and CFTR regulation in the context of cystic fibrosis. This research leverages in vitro airway epithelial models and electrophysiological techniques to investigate the molecular mechanisms of CFTR function and pharmacological modulation. While our lab does not directly aim to develop therapies, our goal is to contribute to the foundational understanding of epithelial signaling and CFTR function in ways that may inform therapeutic innovation. This work connects us with the broader cystic fibrosis research community, particularly through collaborations and scientific exchange supported by the Cystic Fibrosis Foundation (CFF) and the Oklahoma Center for Respiratory and Infectious Diseases (OCRID). We also engage with research presented at the North American Cystic Fibrosis Conference (NACFC) and related professional meetings.

Translational | Mechanisms and Airway Epithelial Ion Transport

This research explores the basic mechanisms that govern ion transport across the airway epithelium, with an emphasis on the cAMP/PKA activated Cl- channel, CFTR. We investigate how cellular and epithelial context, including cell type, ionic environment, and signaling pathways, shapes ion channel function and regulation. Our studies use various cultured human airway epithelial cells and apply electrophysiological and biochemical techniques to analyze regulation of cellular signaling and ion transport activity. We also consider the molecular and functional evolution of the CFTR protein. This work aims to improve our understanding of how epithelial signaling and cellular context influence ion homeostasis in both normal and disease states, including cystic fibrosis (CF).

Ongoing Projects

Selected Papers

ENaC downregulation paper

Down-regulation of the epithelial sodium channel (ENaC) in human airway epithelia in response to low temperature incubation

BMJ Open Respir Res, 2021

Yadav S*, Shaughnessy CA*, Zeitlin PL, Bratcher PE

Chloride concentration and CFTR modulation

Effect of apical chloride concentration on the measurement of responses to CFTR modulation in airway epithelia cultured from nasal brushings

Physiol Rep, 2020

Bratcher PE, Yadav S, Shaughnessy CA, Thornell IM, Zeitlin PL

Polyp-derived cell cultures and modulator response

Nasal epithelial cell cultures from nasal polyps are more responsive to CFTR modulators than cultures from healthy sinuses

J Cyst Fibros, 2020

Shaughnessy CA, Yadav S, Zeitlin PL, Bratcher PE

Translational | Exploring Novel Insights into CFTR Modulation

This research investigates how pharmacological modulators of CFTR affect osmoregulatory function in airway epithelial models. We focus on understanding the mechanisms by which compounds like elexacaftor, tezacaftor, and ivacaftor alter CFTR activity, alone or in combination with novel co-modulators. Using in vitro experimentation, cAMP detection, and electrophysiological assays, we assess synergistic responses, receptor-mediated pathways, and the context-dependence of modulator action across different epithelial models. While this work is exploratory and not directly therapeutic, it aims to provide mechanistic insights that contribute to the broader understanding of CFTR pharmacology in the context improving therapy for of cystic fibrosis.

Ongoing Projects

Selected Papers

Prostaglandin-mediated CFTR activation

Receptor-mediated activation of CFTR via prostaglandin signaling pathways in the airway

Am J Physiol Lung Cell Mol Physiol, 2022

Shaughnessy CA, Yadav S, Bratcher PE, Zeitlin PL

Elexacaftor potentiation study

Elexacaftor is a CFTR potentiator and acts synergistically with ivacaftor during acute and chronic treatment

Sci Rep, 2021

Shaughnessy CA, Zeitlin PL, Bratcher PE

Ivacaftor benefit in prolonged combination therapy

Net benefit of ivacaftor during prolonged tezacaftor/elexacaftor exposure in vitro

J Cyst Fibros, 2022

Shaughnessy CA, Zeitlin PL, Bratcher PE